Quantitative analysis of Terminal Restriction Fragment Length Polymorphism (T-RFLP) microbial community profiles: peak height data showed to be more reproducible than peak area
نویسندگان
چکیده
منابع مشابه
Restriction Fragment Length Polymorphism (RFLP)
Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...
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Terminal restriction fragment length polymorphism (T-RFLP) is a culture-independent method of obtaining a genetic fingerprint of the composition of a microbial community. Comparisons of the utility of different methods of (i) including peaks, (ii) computing the difference (or distance) between profiles, and (iii) performing statistical analysis were made by using replicated profiles of eubacter...
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Rapid analysis of microbial communities has proven to be a difficult task. This is due, in part, to both the tremendous diversity of the microbial world and the high complexity of many microbial communities. Several techniques for community analysis have emerged over the past decade, and most take advantage of the molecular phylogeny derived from 16S rRNA comparative sequence analysis. We descr...
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By using traditional bacterial culture methods, only less than 1% of bacteria from environmental samples can be cultivated for biodiversity and microbial ecology studies. In recent years, however, rapid development in molecular biology and ecology has enabled us to explore genetic diversity of bacteria in environmental samples using culture-independent approaches. For example, the recent emerge...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2007
ISSN: 1517-8382
DOI: 10.1590/s1517-83822007000400027